Cardiovascular effects of PACAP and PACAP(6â38) in the RVLM of normotensive and hypertensive rats. A and B: changes in mean arterial pressure (MAP; i), HR (ii), and percentage of sSNA (iii) before and after the administration of PACAP (A) or PACAP(6â38) (B). Arrows indicate times of drug infusion. âPBSâ indicates the period after the bilateral RVLM microinjection of PBS; âPACAPâ and âPACAP(6â38)â indicate the periods after the bilateral RVLM microinjection of PACAP or PACAP(6â38), respectively. C: comparison of maximum MAP (i), HR (ii), and percentage of sSNA responses (iii) after PACAP or PACAP(6â38). *P < ; **P < ; ***P < .
The mass spectrometric analysis produces a list of molecular weights of the fragments which is often called a peak list. The peptide masses are compared to protein databases such as Swissprot , which contain protein sequence information. Software performs in silico digests on proteins in the database with the same enzyme (. trypsin) used in the chemical cleavage reaction. The mass of these peptide fragments is then calculated and compared to the peak list of measured peptide masses. The results are statistically analyzed and possible matches are returned in a results table.